RNA and protein derived from EVs can be easily and quickly extracted without using an ultracentrifuge. The easy-to-handle spin column type simplifies the work, which can be expected to reduce variability and ensure reproducibility.

Applications

  • Extraction of exosome-derived biomarkers for early diagnosis and treatment selection
  • Biomarker discovery and basic exosome research
  • Body fluids (serum, plasma, urine, etc.) and cell supernatants can be used

Features

  • Three-dimensional porous glass technology with precisely controlled pore size
  • Coating technology that does not adhere contaminant proteins
  • Isolate EVs easily and quickly (15 min)
  • Size separation enables you to capture bias-free EVs

Key technology

Precisely controlled porous glass pore size helps you capture EV-sized particles selectively. By coating the surface of the filter with a non-adhesive protein material, you can refrain from the adsorption of contaminant proteins, such as albumin. In addition, you can obtain highly pure EVs inside the filter by combining them with a washing reagent.


Protocol overview

  • Pretreatment

    Pretreat the specimen with a 0.22 μm syringe filter.

  • EVs capture
  • Input sample (100-500uL) into the column and centrifuge for 10min.

  • Washing
  • Load the washing reagent into the column and centrifuge for 5 min.

  • Extraction
  • Extract RNA and protein from the column using commercially available extraction reagents, and proceed to analysis.

Data example

Exosomal protein markers and purity

EVAGLAX wash buffer A enabled us to obtain CD9 intensity and protein purity equivalent to ultra centrifugation (UC).

  • AGC’s survey

Evaluation of exosomal miRNA concentration

Total RNA concentrations equivalent to the ultracentrifugal method have been obtained under EVAGLAX wash buffer A conditions.

  • AGC’s survey

Protocol link

EVAGLAX_Protocol_v1.0_English

You can watch videos on Youtube. Please read the two-dimensional code or press the link.